Banca de DEFESA: HIANNE CRISTINNE DE MORAIS MEDEIROS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : HIANNE CRISTINNE DE MORAIS MEDEIROS
DATE: 21/10/2020
TIME: 14:00
LOCAL: PLATAFORMA REMOTA - GOOGLE MEET
TITLE:
KEY WORDS:

Benign epithelial odontogenic lesions; Tumor suppressor genes; ING Proteins

PAGES: 92
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

The aim of this study is to evaluate the correlation between expression of proteins of the ING family and expression of protein p53 in dental follicles, odontogenic keratocysts, adenomatoid odontogenic tumors and ameloblastomas. The sample was intentional and non-probabilistic, consisting of 20 cases of ameloblastomas, 20 cases of adenomatoid odontogenic tumors, 20 cases of odontogenic keratocysts and 10 samples of dental follicles. The specimens were subjected to immunohistochemical method, using antibodies anti-Ing1, anti-Ing2, anti-Ing3, anti-Ing4, anti-Ing5 and anti-p53. Quantitative analysis was performed taking into account the cytoplasmic and / or nuclear location for the ING proteins and the nuclear location for the p53 protein. The data were analyzed by the Statistical Package for Social Sciences for Windows (SPSS version 22.0; IBM, USA). The Kruskal Wallis test was used to compare the immunoexpression between the groups of lesions, and the Spearman test was used to investigate the correlations. Values of p ≤ 0.05 were considered significant. The results of this study showed a reduction in ING3 expression in ameloblastomas (p = 0.003); a reduction in ING4 expression, in odontogenic keratocysts (p = 0.02) and in ameloblastomas (p = 0.001); and a reduction in the ING5 nuclear expression, also in odontogenic keratocysts (p = 0.09) and in ameloblastomas (p = 0.012). Positive correlations were found between p53 nuclear expression with ING1 cytoplasm / nucleus expression (r = 0.603; p = 0.05) in odontogenic keratocysts, and with ING3 cytoplasm / nucleus expression (r = 0.475; p = 0.034) and also ING4 cytoplasm / nucleus expression (r = 0.448; p = 0.047) in ameloblastomas. Finally, this study provided evidence that reduction INGs expressions seems to be an event involved in the etiopathogenesis of more aggressive odontogenic lesions, and that the nuclear / cytoplasmic expression of ING1, ING3 and ING4 is related to the lower p53 expression in odontogenic keratocysts and ameloblastomas, which indicates that these proteins seem to influence the cell cycle in a p53-dependent manner in these lesions.

BANKING MEMBERS:
Externa à Instituição - DENISE HELEN IMACULADA PEREIRA DE OLIVEIRA - UFC
Interna - 2492713 - ERICKA JANINE DANTAS DA SILVEIRA
Externo à Instituição - GUSTAVO PINA GODOY - UFPE
Presidente - 1258693 - LELIA MARIA GUEDES QUEIROZ
Interno - 2859541 - PEDRO PAULO DE ANDRADE SANTOS