Banca de DEFESA: MARIANA CABRAL MORENO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIANA CABRAL MORENO
DATE: 25/09/2024
TIME: 14:00
LOCAL: Departamento de Odontologia - Auditório
TITLE:

EFFECT OF GREEN PROPOLIS EXTRACT IN DIFFERENT CONCENTRATIONS ON ORTHODONTIC MOVEMENT IN RATS

KEY WORDS:

Orthodontics; Tooth movement techniques; Rats; Propolis.

PAGES: 38
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUBÁREA: Ortodontia
SUMMARY:

Green propolis, a widely consumed natural substance, has demonstrated beneficial effects on bone tissue. This study aimed to evaluate the effect of alcoholic green propolis extract at different concentrations on orthodontic tooth movement (OTM) in rats. The experiment followed the Animal Research Reporting In Vivo Experiments (ARRIVE) guidelines and included 40 (after the losses, the sample consisted of 40 animals healthy male Wistar rats (250g), aged 7-10 weeks, subjected to OTM using a closed nickel-titanium coil spring (50cN) placed between the first upper left molar and the maxillary incisors, with daily administration of substances via gavage for 21 days. The rats were divided into four groups: control (GC, water), experimental group 1 (P1, propolis 100 mg/kg), experimental group 2 (P2, propolis 300 mg/kg), and experimental group 3 (P3, propolis 500 mg/kg). Green propolis extract was analyzed by high-performance thin-layer chromatography (HPTLC) and UV-VIS spectrophotometric scanning to determine flavonoids, caffeic acid, and total phenolic compounds. The systemic effect of green propolis was evaluated in the animals, showing no interference with OTM. Micro-computed tomography (Micro-CT) was used to analyze linear OTM (mesial and vertical movement, and periodontal ligament thickness in the tension and pressure areas) and volumetric changes (bone volume %, bone porosity %, trabecular number, and trabecular spacing), using one-way analysis of variance (ANOVA) followed by Tukey’s post-test (5%), with no significant differences (p>0.05). Histological analysis of the distobuccal root revealed bone remodeling in the tension (distal side) and pressure (mesial side) areas, with osteoblastic activity present in all groups on the tension side. A significant difference (p<0.05) was observed on the pressure side, with more pronounced inflammatory modulation in the propolis-treated groups, as determined by Kruskal-Wallis followed by Dunn’s post-test (5%). The study suggests that green propolis has the potential to modulate inflammation and preserve bone quality without compromising tooth movement

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Externo à Instituição - ALEXANDRE ANTONIO RIBEIRO - ABO
Externo à Instituição - ARY DOS SANTOS-PINTO - UNESP