Banca de DEFESA: RANI IANI COSTA GONÇALO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : RANI IANI COSTA GONÇALO
DATE: 17/03/2023
TIME: 14:00
LOCAL: REMOTO
TITLE:

The influence of Yes-associated protein (YAP) on cell proliferation and apoptosis in benign epithelial odontogenic lesions


KEY WORDS:

Yes-associated protein. Cell proliferation. Apoptosis. Odontogenic cysts. Odontogenic tumors. Immunohistochemistry.


PAGES: 121
BIG AREA: Ciências da Saúde
AREA: Odontologia
SUMMARY:

Background: Odontogenic cysts and tumors present heterogeneous biological behavior, and their etiopathogenesis is not fully understood yet. Yes-associated protein (YAP) acts as a transcriptional regulator of genes involved in cell proliferation and apoptosis, activating pathways associated with cystic growth and neoplastic progression. Objective: To analyze the immunohistochemical expression of YAP protein and correlate it with markers involved in cell proliferation and apoptosis in benign epithelial odontogenic lesions. Methods: The sample consisted of 95 cases of odontogenic lesions - 25 dentigerous cysts (DCs), 30 non-syndromic OKCs (OKCs), 30 conventional AMB (C-AMBs), and 10 unicystic AMB (U-AMBs) -, in addition to 10 specimens of dental follicles (DF). Clinicodemographic data collection was carried out, as well as morphological analysis for better characterization of the sample. The histological sections were submitted to the immunohistochemical technique using YAP, cyclin D1, Ki-67, and Bcl-2 antibodies, and their immunoexpression analysis was performed through an adapted methodology. The collected data were submitted for descriptive and statistical analysis (p ≤ 0.05). Results: There was a slight predilection for women (n = 55; 57.6%) and individuals aged between 21 and 40 years (n = 50; 47.6%), with the posterior region of the mandible as the most affected site (64%). Except for DFs, in all groups of lesions, there was a predominance of a more densely collagenized capsule/stroma (n = 81; 77.15%). Analysis of YAP immunoexpression revealed higher expression levels in OKCs, especially in the basal and parabasal layers, followed by U-AMBs and C-AMBs, which showed moderate immunoreactivity, predominantly in peripheral cells. In addition, there were significant differences in YAP immunoexpression between the analyzed groups, with positive and statistically significant correlations between YAP and cyclin D1 in DCs and U-AMBs, and between YAP and Ki-67 in U-AMBs (p < 0.05). However, between YAP and Bcl-2 immunoexpression, there was no statistically significant correlation. Conclusions: YAP influences the cell proliferation of odontogenic cysts and tumors epithelium, thus helping with the progression of the different odontogenic lesions.


COMMITTEE MEMBERS:
Externa ao Programa - 3218128 - AMANDA KATARINNY GOES GONZAGA - nullExterna à Instituição - DENISE HELEN IMACULADA PEREIRA DE OLIVEIRA - UFC
Interna - ***.887.244-** - LELIA BATISTA DE SOUZA - UFRN
Presidente - 1258693 - LELIA MARIA GUEDES QUEIROZ
Externo à Instituição - LEORIK PEREIRA DA SILVA - UFCG
Notícia cadastrada em: 16/03/2023 11:12
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