Banca de QUALIFICAÇÃO: MARIA GABRIELA PAZ DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA GABRIELA PAZ DA SILVA
DATE: 23/08/2024
TIME: 10:00
LOCAL: Plataforma Google Meet
TITLE:

Electrophysiological Evaluation of Cochlear Synaptopathy Related to Aging in Rodents

KEY WORDS:

Aging; Cochlear Synaptopathy; Rats.

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Fonoaudiologia
SUMMARY:

In the natural aging process, there is a reduction in synapses between the inner hair cells and the neurons of the spiral ganglion of the auditory nerve, known as cochlear synaptopathy (CS). Studies in rats have proposed the use of short-latency auditory evoked potentials to identify CS, but there are gaps as to the best parameters for clinical application. In this context, the aim of this study is to assess the applicability of short-latency auditory evoked potentials in identifying age-related CS in rodents. The dissertation was structured in two manuscripts, the first being a systematic review of the literature with the aim of synthesizing data that measures the sensitivity of short-latency auditory evoked potentials in rodents. The research was carried out in accordance with the recommendations of the Preferred Reporting Items for a Systematic Review and Metaanalysis of Diagnostic Test Accuracy Studies (PRISMA-DTA), with a search in the PUBMED/MEDLINE, Cochrane Library, Scopus, Embase, Web of Science, Scielo, LILACS and gray literature databases. The articles were selected by two reviewers, independently and blindly, using the Rayyan platform to manage the studies. The second manuscript consists of a primary observational and longitudinal study, with the aim of verifying the influence of the brainstem auditory evoked potential stimulation rate on the identification of CS in aging. Sixteen Wistar rats of both sexes will take part in the study, divided into two groups: eight young adult rats aged between 3 and 4 months (G1) and eight middle-aged adult rats aged 10-13 months (G2), which will be submitted to brainstem auditory evoked potentials at different stimulation rates. After the first assessment, half of the sample will undergo immunohistochemical analysis and the others will be reassessed longitudinally using the same electrophysiological and immunohistochemical assessment protocol, i.e. G1 at 9-10 months and G2 between 16 and 19 months, which is the beginning of ageing. We hope to gather data that will allow us to quantify the sensitivity of short latency auditory potentials in identifying CS, as well as suggesting a stimulation rate with greater signal reliability to identify CS.

COMMITTEE MEMBERS:
Presidente - 2358823 - ELIENE SILVA ARAUJO
Interna - ***.092.134-** - KELLY CRISTINA LIRA DE ANDRADE - UNCISAL
Externa à Instituição - KÁTIA DE FREITAS ALVARENGA - USP