Banca de DEFESA: MARIA JOSE SARMENTO FILHA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MARIA JOSE SARMENTO FILHA
DATA : 05/12/2018
HORA: 14:00
LOCAL: Sala de aula do PPgDITM
TÍTULO:

AVALIAÇÃO DA COMPOSIÇÃO QUÍMICA E POTENCIAL BIOLÓGICO DAS FLORES, FOLHAS, CAULES, FRUTOS E SEMENTES DA ESPÉCIE VEGETAL Cochlospemum vitifolium (PACOTÊ) 

PALAVRAS-CHAVES:

Anti-inflammatory; antioxidant; Cochlospermum vitifolium; leishmanicide; Pacotê;

PÁGINAS: 131
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The Cochlospermum vitifolium specie, popularly known as pacotê, is a
medicinal plant used of treatment of hypertension, hepatitis, jaundice, hyperglycemia
and inflammatory disorders, in addition have increase menstrual flux property, that
can be attributed the presence of chemical compounds of species. However, not
have chemical and biological studies with isolated parts of the plant, so, the study
aimed to identify chemical compounds present in ethanolic extract, hexane, ethyl
acetate and methanol/water fractions of C. vitifolium flowers, leaves, stalks, fruits and
seed and evaluate the antioxidant and leishmanicide effect in vitro, as well as
evaluate anti-inflammatory potential of ethanolic extract and ethyl acetate fraction of
flowers in vivo experimental models. The chemical profile of ethanolic extract and
ethyl acetate fractions of flowers, leaves and stalks of C. vitifolium was evidenced by
UPLC-QTOF-MS/MS. The compounds of flowers were tentantly identified as
hydrolysable tannins (galotannins, galoylquinones, ellagitannins), flavonoids,
phenolic derivatives of polyamide spermidine, hydroxylated fatty acids, in addition to
the compounds: quinic acid, syringic acid, cinnamic acid dihexoside and arjunolic
acid. For the stalks, besides some compounds identified of flowers the prunin,
taxifoline, aromadendrin, naringenin and kaenferol were identified. The total phenolic
content was express in equivalents of gallic acid per gram of sample, highlighting the
ethyl acetate fraction (525.90 mg EAG/g). The antioxidant potential was evaluated for
diphenylpicryl hydrazine (DPPH), total antioxidant capacity, iron and cooper
chelating, reducing power, superoxide (O2-) and hydroxyl (OH-) radical scavenging
assays, demonstrating the greater anti-radical activity against the radical DPPH (IC50
= 5.03 ± 0.10), chelation of copper ions of 84.26% and sequestration of the O2-
radical with 99.18% at 0.05 mg/mL for ethyl acetate fraction. The ethanolic extract
and fractions of flowers, leaves, stalks and fruits showed reductive capacity dose
dependent, with reducing power above 100% by 0.25 mg/mL. The methanolic
fraction from the fruits showed greater capacity antioxidant against the (OH-) radical
(39.84% in 0.3mg/mL). The best ability to chelate iron ions (52.27% in 0.3mg / mL).
The leishmanicide activity was evaluated for promastigotes forms, which the hexanic
fraction from the leaves and stalks were capable of inhibit parasite growth. The
ethanolic extract and ethyl acetate fraction from the flowers of C. vitifolium
demonstrated anti-inflammatory activity in vivo experimental models. The results
revealed the presence of important chemical compounds in samples of C. vitifolium,
which could be responsible for the antioxidant, leishmanicidal and anti-inflammatory
activity, revealing a therapeutic potential for the development of new molecules
phytotherapeutic with anti-inflammatory activity.

MEMBROS DA BANCA:
Presidente - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externo ao Programa - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 2276354 - LEANDRO DE SANTIS FERREIRA
Externo à Instituição - RAQUEL DE MELO BARBOSA
Externo à Instituição - YANNA CAROLINA FERREIRA TELES - UFPB