Banca de DEFESA: GIOVANI JARDINI LAVIERI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GIOVANI JARDINI LAVIERI
DATE: 10/06/2025
TIME: 10:30
LOCAL: VIDEOCONFERÊNCIA
TITLE:

The role of miRNAs in Lyme disease: a review

KEY WORDS:

Lyme disease, miRNA, Pathogenesis, Regulatory pathways, Borrelia burgdorferi, Lyme arthritis, Lyme carditis, Neuroborreliosis.

PAGES: 78
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Introduction: Lyme disease (LD) is the most common tick-borne disease in North America

and Europe. Caused by the bacterium Borrelia burgdorferi, it manifests itself in different forms

and stages, and may be asymptomatic or with nonspecific symptoms, in addition to the

difficulty in localizing the arachnid bite and the possible lack of formation of erythema

migrans, which makes clinical diagnosis difficult. Current laboratory diagnostic methods have

limitations, such as the absence of antibodies against bacteria during the first weeks of

infection, in addition to access to samples, since Borrelia burgdorferi lodges in difficult-to

access tissues. In this context, miRNAs emerge as potential candidates in understanding the

pathophysiology of LD, as well as biomarkers and therapeutic targets, given their stability and

ability to reflect pathological states, including bacterial infections. Objective: To conduct a

narrative review on the role of miRNAs in LD. Methodology: The search was performed in

PubMed, Embase, ScienceDirect, Web of Science and LILACS databases, using the terms

"Lyme Disease", "Borrelia burgdorferi", "Lyme Borreliosis", "Lyme Arthritis", "Lyme

Carditis" and "MicroRNA". Results: Of the 7 studies included, three were performed in vitro

with different human cell cultures, two used in vivo models with mice, one study was

performed in vitro with Ixodes scapularis ticks and one was observational with patient samples.

Total RNA extraction methodologies varied between the TriZol method and the miRNeasy Kit

and the analysis of miRNA expression varied from RNAseq to qRT-PCR. In total, 261

differently expressed miRNAs were analyzed among all studies and, of these, miR-146a-5p,

miR-155, miR-145 and miR-146b were described in more than one study with increased

expression in different samples and clinical manifestations. Conclusion: miRNAs such as

miR-146a-5p, miR-155, miR-145, and miR-146b play important roles in the inflammatory

regulation of LD, being involved in dermal manifestation, arthritis, carditis, and microglial

activation. These miRNAs have potential as biomarkers and therapeutic targets in LD, although

further studies are needed to validate their applicability in the diagnosis and treatment of the

disease.

COMMITTEE MEMBERS:
Interna - 1055045 - MARCELA ABBOTT GALVAO URURAHY
Externo à Instituição - VICTOR HUGO REZENDE DUARTE - UNIFACEX
Presidente - 1804884 - VIVIAN NOGUEIRA SILBIGER