Banca de DEFESA: HÉLIDA MARAVILHA DANTAS E SOUSA ALMEIDA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : HÉLIDA MARAVILHA DANTAS E SOUSA ALMEIDA
DATE: 19/12/2024
TIME: 09:00
LOCAL: Link de acesso para videoconferência:https://meet.google.com/erh-bnvu-obz
TITLE:
KEY WORDS:
Phytol; Nanotechnology; Nanoemulsion; Anxiety.
PAGES: 180
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:
Phytol, a diterpene with notable effects on the central nervous system, has
demonstrated anxiolytic properties in Swiss mice subjected to the elevated plus maze.
Despite these findings, limited research has explored the impact of phytol
nanoemulsions in animal models of anxiety, particularly in females. The present study
aims to evaluate the effects of phytol incorporated into a sunflower oil nanoemulsion
on rats exposed to the elevated plus maze. The formulation was developed using the
phase inversion method, varying phytol concentration within the oil phase. The most
stable formulation, containing the highest diterpene concentration, underwent
extensive physicochemical characterization, including droplet size, polydispersity
index (PDI), zeta potential, and pH over 35 days. Characterization techniques included
Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy
(TEM), and chromatographic analysis. Additionally, the formulation was tested for
hemolytic activity and antioxidant capacity using the DPPH assay and evaluated for its
biocompatibility and delivery to the brain using a chicken embryo model. In vivo studies
were performed in Wistar rats, with the animals divided into treatment groups (vehicle;
diazepam 2 mg/kg; phytol 25 mg/kg; phytol 75 mg/kg; blank nanoemulsion; phytol
nanoemulsion 25 mg/kg; and phytol nanoemulsion 75 mg/kg) administered via
intraperitoneal injection. The physicochemical analysis revealed that the highest
achievable phytol concentration in the nanoemulsion was 2.5%, with an average
droplet size of approximately 160 nm, a PDI of 0.235, and a negative zeta potential of
-27 mV, showing stability for over 30 days. Behaviorally, a significant reduction in
anxiety-like behaviors was observed, as evidenced by increased entries and time
spent in the open arms of the maze for rats treated with both the blank and phytol
nanoemulsions at varying concentrations, compared to the vehicle group and the free
phytol treatment at 25 mg/kg. These findings suggest that the phytol nanoemulsion
formulation exhibits superior anxiolytic efficacy compared to the free phytol,
highlighting the potential therapeutic advantage of the nanoemulsion system.
COMMITTEE MEMBERS:
Externa ao Programa - 2121895 - ALIANDA MAIRA CORNELIO DA SILVA - nullPresidente - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externo à Instituição - DANIELA BAPTISTA DE SOUZA - UNESP
Interno - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA