Banca de DEFESA: JULIANA BARRETO PEREIRA DE SOUZA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : JULIANA BARRETO PEREIRA DE SOUZA
DATE: 04/11/2025
TIME: 14:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

Development and characterization of inclusion complexes of Rosemary-Pepper

essential oil (Lippia origanoides Kunth)

KEY WORDS:

Antimicrobial. Antioxidant. Cyclodextrin.

PAGES: 220
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

Rosemary pepper essential oil (Lippia origanoides) already has microbiological and antioxidant activities listed in the literature that correspond as a great alternative for the cosmetic industry. However, the use by industry is limited due to volatility. Alternatively, inclusion complexes have been used to increase the solubility and stability of oils without changing their properties. Therefore, the present work proposes to obtain and physicochemical characterization of the inclusion complex with essential oil in β-cyclodextrin (β-CD) and hydroxypropyl-βcyclodextrin (HP-β-CD), and analyze them for their antimicrobial and antioxidant activity, always comparing them with the essential oil. The chemical characterization of the essential oil was carried out by gas chromatography coupled to mass spectrometry (GC-EM), obtaining 64,55% of thymol as the majority compound and molar mass of 150.221 g/mol. The encapsulation efficiency of the complexes produced by the malaxing (M) and slurry (S) methods showed that the HP-β-CD complexes were the lowest loss of essential oil during the process, being less than 3%. The chemometrics of essential oil highlighting the comparison between batches, indicating that even in the same period of the year and with a stable climate, it is possible to find variations between batches, showing the importance of this characterization by batch, as it can lead to different properties. They were also characterized by FTIR, SEM, ED-XRF, DR-X, NMR, TG and DSC, demonstrating that the complexation between essential oil and cyclodextrins (β-cyclodextrin and hydroxypropyl-β-cyclodextrin) is a mechanism of thermally stabilize the oil and be able to incorporate it into cosmetics formulations without losing properties, since the complexes aggregated the characteristics of cyclodextrins and only began to show mass loss (indicating degradation of the constituents) at high temperatures. The evaluation of the antifungal activities showed activity for the essential oil and the inclusion complexes in all tested strains, with a maximum concentration of 128 µg/mL for the most resistant strains. Conversely, the analysis of antibacterial activity revealed that the essential oil has a minimum antibacterial activity of 32 μg/mL in all tested strains, whereas the complexes showed no activity. In the study of antioxidant activity, it was possible to determine that the essential oil exerts action in all studied antioxidant mechanisms, although the complexes only yielded positive results in the mechanisms of superoxide radical scavenging, copper chelation, and reducing power. When in vitro antioxidant activity was assessed using MRC-5 cells, the essential oil exhibited an activity of 41.74%, while the complexes showed no activity. On the other hand, the two complexes with HP-β-CD and the essential oil did not show cytotoxicity. This project aims to enhance the local biome (Caatinga), adding ecological value to the biome, favoring its conservation, with Lippia origanoides being a relevant resource for sustainable use and scientific appreciation of this biome. Furthermore, this work has resulted in new perspectives and alternatives for the incorporation of essential oil in pharmaceutical formulations, contributing to the strengthening of the pharmaceutical and cosmetic production chain.

COMMITTEE MEMBERS:
Presidente - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externo à Instituição - BOLIVAR PONCIANO GOULART DE LIMA DAMASCENO - UEPB
Externo ao Programa - 2140814 - FERNANDO HENRIQUE ANDRADE NOGUEIRA - nullInterna - 1871916 - RAQUEL BRANDT GIORDANI
Externo à Instituição - RICARDO NEVES MARRETO - UFG