Banca de DEFESA: GILSON CASSIANO DE GÓES NETO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GILSON CASSIANO DE GÓES NETO
DATE: 31/01/2025
TIME: 14:00
LOCAL: VIDEOCONFERÊNCIA
TITLE:

IN VITRO ANTIVIRAL EVALUATION OF NEW DELIVERY SYSTEMS WITH NAPHTHOQUINONE DERIVATIVE

KEY WORDS:

DENV; dengue; naphthoquinones; IVS320; cyclodextrins; inclusion complexes; drug delivery systems; pharmaceutical technology

PAGES: 90
BIG AREA: Ciências da Saúde
AREA: Farmácia
SUMMARY:

The outbreak of dengue fever, a disease transmitted by the DENV virus, has spread globally, causing

millions of deaths worldwide. Therefore, it is necessary to develop new compounds that can act in the

treatment of dengue fever. Naphthoquinones (NQs) and their derivatives have antifungal, antiparasitic

and antimicrobial action, among which is the compound IVS320, a molecule with promising

antimicrobial action, but with low solubility. Cyclodextrins (CDs) are used as a strategy to improve the

solubility, stability and bioavailability of drugs. In this context, the development of inclusion

complexes (ICs) with cyclodextrins can solve the implicit restrictions of IVS320, and in turn, increase

its bioavailability and pharmacological action. Thus, the present study aims to improve the

physicochemical and biological properties of naphthoquinone IVS320, through the development of

inclusion complexes. In addition to evaluating the in vitro anti-DENV activity of the obtained delivery

systems and analyzing the bioactive potential of the compound, the complexes obtained with γ-CD,

HP-γ-CD and HP-β-CD, through the physical mixing ( MF), kneading (ML) and rotary evaporation

(RT) techniques, were characterized by XRD, FTIR, DSC and TG techniques. The XRD results of the

isolated IVS320 exhibited crystalline reflections with high intensity at 10°, 18°, 32° and 37°, while the

diffractograms of the MF, ML and RT systems showed a reduction in the crystalline profile,

suggesting the formation of complexes . The FTIR spectra of the systems bands showed characteristic

of both IVS320 and the CDs employed, with modifications in the profile of some bands. In turn, the

DSC showed little similarity with the endothermic and exothermic events observed in the isolated

IVS320, and the IC exhibited the main occurrences in the ranges of 70-80°C, 190-200°C, up to

exothermic peaks in the range of 250°C-260°C, signaling crystallization. While in the TG, well

defined stages of mass loss were observed for all complexation techniques, in which the first events of

the systems occurred around 50°C to 70°C, with approximately 10% mass loss, while the second

variation began between 300 and 340°C, followed by gradual mass decay up to 600°C, with the

highest ∆m (approximately 80%). Regarding in vitro activity, the compound IVS320 presented quite

satisfactory results, being able to inhibit the DENV virus. The results of the physicochemical

characterization indicated the formation of IVS320 ICs with CDs, demonstrating that the methods

employed were effective and that the systems developed are promising for enhancing the antiviral

activity of IVS320.

COMMITTEE MEMBERS:
Presidente - 1789788 - ADLEY ANTONINI NEVES DE LIMA
Externo à Instituição - FABIO ROCHA FORMIGA - FIOCRUZ
Interno - 2275890 - MARCELO DE SOUSA DA SILVA