Banca de QUALIFICAÇÃO: LAYSA OHANA ALVES DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LAYSA OHANA ALVES DE OLIVEIRA
DATE: 28/01/2022
TIME: 14:00
LOCAL: Videoconferência
TITLE:

In silico analysis of transcriptome of GG-NER and TC-NER deficient cells in response to oxidative stress

KEY WORDS:

Oxidative stress; Nucleotide excision repair (NER); GG-NER; TC-NER; CSB; XPC.

PAGES: 82
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The nucleotide excision repair (NER) pathway removes bulky lesions, such as UV radiation products (pyrimidine dimers and 6.4 photo-products), benzo[a]pyrene adducts and damage caused by
chemotherapy. The NER pathway has shown its importance in repairing DNA damage caused by oxidation next to the BER pathway, and can be used in the absence of BER. This pathway is
divided into two sub-pathways, the transcription-coupled repair pathway (TC-NER), and the global genome repair pathway (GG- NER); The TC-NER pathway is responsible for the defense of the
genetic material that is being transcribed, while the GG-NER repairs lesions in any part of the genome. Syndromes such as Cockayne (CS), Xeroderma Pigmentosum (XP) and trichotiodistrophy (TTD) are some of the consequences caused by genetic mutations in the NER pathway, mutations that cause sensitivity to UV light and predisposition to cancer, which are some of the symptoms present in patients syndromes mentioned above and are correlated with deficiency in the NER pathway. Syndromes such as XP and CS present mutations in genes involved in the NER pathway and respond to oxidative stress with the most varied phenotypes. Thus, the present work analyzed the transcriptome of cells deficient in CSB (CSIAN) and XPC (XP4PA), cell lines deficient in TC-NER and GG-NER, respectively, treated with hydrogen peroxide (H2O2). The analysis was performed using
computational tools, such as Cytoscape, Panther and MIENTURNET, in order to predict interactions performed by these proteins that can elucidate the different phenotypes in response to oxidative stress. The result of the analysis led to the hypothesis that CSB and XPC are interacting with proteins involved in processes not associated with repair, such as MAPK1, JUN, HDAC1, PIK3CD, TAF1, ELK1 and others. Surprisingly, XP4PA cells showed a large number of up-regulated miRNAs in response
to oxidative stress, suggesting a role for XPC in the regulation of these transcripts.

BANKING MEMBERS:
Presidente - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN
Interna - 1453487 - KATIA CASTANHO SCORTECCI
Interna - 1199127 - SILVIA REGINA BATISTUZZO DE MEDEIROS