Banca de DEFESA: MYCHELLE DE MEDEIROS GARCIA TORRES

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MYCHELLE DE MEDEIROS GARCIA TORRES
DATE: 23/08/2025
TIME: 14:00
LOCAL: Centro de Biociências
TITLE:

Diagnostic integration for endometrial analysis in infertility: video hysteroscopy, immunohistochemistry and molecular diagnosis.

 

KEY WORDS:

Endometrium, endometritis, infertility, microbiome, microbiota.

PAGES: 96
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Inflammatory alterations and imbalances in the endometrial bacteriome have been increasingly recognized as relevant factors in the pathophysiology of female infertility, particularly in cases classified as unexplained infertility. Given the diagnostic complexity involved, this thesis aimed to investigate the relationship between bacterial dysbiosis and endometrial inflammation by integrating hysteroscopic, immunohistochemical, and molecular approaches in order to improve the identification of chronic endometritis and microbiota-related disturbances within the uterine environment. Chapter I presents a critical review of the methodologies used to analyze the endometrial bacteriome, proposing guidelines for protocol standardization and improved reproducibility across studies. Factors such as aseptic sample collection, menstrual cycle phase, DNA integrity, and the use of updated microbial databases were highlighted as essential for robust analysis. Chapter II describes an observational study assessing the prevalence of chronic endometritis in women with unexplained infertility compared to fertile controls. The investigation involved videohysteroscopy-guided endometrial biopsy during the luteal phase and immunohistochemical (IHC) staining for CD138. Results revealed a significantly higher prevalence of chronic endometritis in infertile women, underscoring the role of inflammation in infertility. Chapter III investigated the presence of dysbiosis-associated bacteria using conventional PCR and nested PCR in endometrial biopsy samples. Nested PCR demonstrated significantly higher sensitivity, revealing a high rate of pathogen co-detection in women with inflammatory signs—even in cases classified as negative by traditional methods. The correlation between dysbiosis and chronic endometritis suggests that inflammation, more than fertility status, is associated with the presence of multiple pathogens. Together, the three chapters emphasize the importance of integrating diagnostic tools and considering the inflammatory context of the endometrium for a more accurate assessment of infertility. The application of sensitive molecular techniques represents a promising advancement for early diagnosis and personalized treatment of uterine conditions within reproductive medicine.

 

 

COMMITTEE MEMBERS:
Externa à Instituição - AMALIA CINTHIA MENESES DO REGO - UnP
Interno - 1880243 - DANIEL CARLOS FERREIRA LANZA
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externo à Instituição - RICARDO NEY OLIVEIRA COBUCCI - EBSERH
Interna - 2085604 - SUSANA MARGARIDA GOMES MOREIRA