Banca de DEFESA: CAIO RODRIGUES MAIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAIO RODRIGUES MAIA
DATA : 11/08/2022
HORA: 14:30
LOCAL: DEPARTAMENTO DE ODONTOLOGIA-AUDITORIO
TÍTULO:

Use of Cell Morphometry in the Neoplastic Microenvironment and Its Relation to Interleukin-4 Immunoexpression

PALAVRAS-CHAVES:

Nuclear Morphometry; Cell Morphometry; Oral Squamous Cell Carcinoma; IL-4; Immunohistochemistry.

PÁGINAS: 72
RESUMO:

Cell morphometry is a type of quantitative analysis that uses geometric measurements of cells to obtain information about the nuclear, cytoplasmic and general morphology of cells. Such parameters may be altered due to physiological and pathological processes that modify normal cell morphology, being a relevant analysis in the prognosis of several injuries. Keratinocytes have a polyhedral morphology with ovoid nuclei, but may undergo changes with pathological and physiological events, for example, in the nucleus. These changes may be present in malignant neoplasms, such as squamous cell carcinoma. Interleukin-4 (IL-4) is a cytokine secreted by several types of cells, being involved in the development and differentiation of Th2 cells, acting in the anti-inflammatory response and its expression seems to be related to the development of some neoplasms, including the oral cancer. The objective of the research was to perform cellular morphometric analysis (nuclear and cytoplasmic compartments), identification of nuclear irregularities of malignant keratinocytes and compare with the immunoexpression of IL-4 and depth of invasion in cases of Epidermoid Carcinoma of the Lower Lip (CELI) and Oral Tongue. (CELLO). Thirty cases of each lesion were analyzed. For cell morphometry, 16 malignant keratinocytes were analyzed per case; IL-4 immunoexpression was analyzed in the parenchyma and stroma of the lesions, using image analysis software. The statistical tests of Analysis of Variance, Kruskal-Wallis and Spearman's Correlation were applied. The immunoexpression of IL-4 in the parenchyma was higher in cases of CELI in all analyzed fields (p=0.010; p=0.048; p=0.004); There was a positive correlation between total cell area and depth of invasion (p=0.038) and a negative correlation between IL-4 immunoexpression in the superficial parenchyma and superficial nuclear perimeter (p=0.007). Therefore, it is suggested that there is a protective relationship between IL-4 immunoexpression and the lesions analyzed, as well as morphometric alterations of malignant keratinocytes with IL-4 immunoexpression in these lesions.

MEMBROS DA BANCA:
Interno - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Externa à Instituição - KEILA MARTHA AMORIM BARROSO - UFPB
Presidente - 2859541 - PEDRO PAULO DE ANDRADE SANTOS