Obtaining the prolyl endoprotease enzyme from Aspergillus sp. in malt bagasse and comparison with similar commercial enzyme in the production of gluten-free beer
beer, gluten, prolyl endopeptidase, craft beer, celiac disease
The beer consumer market has been presenting a change in Brazil with the search for differentiated products and with higher quality, the craft beers. However, beer is a drink that contains gluten and can not be consumed by people with celiac disease. In addition, the search for gluten-free foods by people who do not have the disease has shown a considerable increase due to the search for a healthier lifestyle since gluten-free foods have a low caloric content. Thus, the search for ways to produce beer without the presence of gluten, but maintaining its raw materials base (water, barley malt, hops and yeast) has been the subject of research. In this context, the present study aims to produce the enzyme prolil endoprotease from Aspergillus sp. com malt bagasse as a substrate as well as compare with a similar commercial enzyme produced from Aspergillus niger (AN-PEP) in the production of a gluten-free beer aimed at optimizing the temperature and concentration of the same. In addition, a gluten-free beer will be produced with the commercial enzyme, which will be characterized and after sensory analysis, will be compared to beer obtained with gluten under the same conditions.